Hotspot

ycf15-trnL-CAA

IRb · 95528-95847 · 320 bp

Back to Cheirodendron

Pi 0.0125

MarkerSeek score 60.10

Primer available yes

Label ycf15-trnL-CAA

Feature Metadata

label_name: ycf15-trnL-CAA
feature_type: igs
region: IRb
length_bp: 320
variable_sites: 4
pi: 0.0125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-trnL-CAA_p1	ACCCGACAGTACCCATTTTG	AAATGGTAGACACGCGAGAC	50.0	50.0	57.8	58.0	435 bp	1.0	79.1
ycf15-trnL-CAA_p2	ACCCGACAGTACCCATTTTG	TGAAATGGTAGACACGCGAG	50.0	50.0	57.8	58.0	437 bp	1.0	79.1
ycf15-trnL-CAA_p3	ACCCGACAGTACCCATTTTG	GCCTTGGTGGTGAAATGGTA	50.0	50.0	57.8	58.1	447 bp	1.0	78.5
ycf15-trnL-CAA_p4	TATTGAATTCACCCGACCCG	AAATGGTAGACACGCGAGAC	50.0	50.0	57.7	58.0	450 bp	1.0	78.1
ycf15-trnL-CAA_p5	TATTGAATTCACCCGACCCG	TGAAATGGTAGACACGCGAG	50.0	50.0	57.7	58.0	452 bp	1.0	78.1