Hotspot

rpl20-rps12

LSC · 71352-72128 · 777 bp

Back to Cheirodendron

Pi 0.00129

MarkerSeek score 59.50

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 777
variable_sites: 1
pi: 0.00129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	896 bp	1.0	73.3
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	895 bp	1.0	73.3
rpl20-rps12_p3	CTTGAATGAGCCCCTCGAAA	AGCGTCGAGGAACATGTACT	50.0	50.0	57.9	58.8	895 bp	1.0	72.6
rpl20-rps12_p4	TTGAATGAGCCCCTCGAAAG	AGCGTCGAGGAACATGTACT	50.0	50.0	57.9	58.8	894 bp	1.0	72.6
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	GCGTCGAGGAACATGTACTA	50.0	50.0	57.9	57.2	893 bp	1.0	72.5