Hotspot

trnE-TTC-trnT-GGT

LSC · 32857-33663 · 807 bp

Back to Cheirodendron

Pi 0.00248
MarkerSeek score 60.20
Primer available yes
Label trnE-TTC-trnT-GGT

Feature Metadata

label_name
trnE-TTC-trnT-GGT
feature_type
igs
region
LSC
length_bp
807
variable_sites
2
pi
0.00248
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-TTC-trnT-GGT_p1 TTTCGTAGTACCCTACCCCC ACAATAGCGAATCTAATGAATGATTCA 55.0 29.6 57.8 57.7 266 bp 1.0 40.3
trnE-TTC-trnT-GGT_p2 CGTTGCCTCCTTGAAAGAGA ACAATAGCGAATCTAATGAATGATTCA 50.0 29.6 57.8 57.7 229 bp 1.0 40.3
trnE-TTC-trnT-GGT_p3 ACAATATACAATAGCGAATTAATA TCGATAACTTCTTGATCCCTCTTC 20.8 41.7 50.2 57.8 254 bp 1.0 40.3
trnE-TTC-trnT-GGT_p4 ATACAATATACAATAGCGAATTAA TCGATAACTTCTTGATCCCTCTTC 20.8 41.7 50.2 57.8 256 bp 1.0 40.3
trnE-TTC-trnT-GGT_p5 TACAATATACAATAGCGAATTAAT TCGATAACTTCTTGATCCCTCTTC 20.8 41.7 50.2 57.8 255 bp 1.0 40.3