Hotspot

rpl16

LSC · 79980-81456 · 1477 bp

Back to Chasmanthium

Pi 0.00204

MarkerSeek score 60.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1477
variable_sites: 3
pi: 0.00204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCAGAAAGAAAAACCTGGGG	TTCCCGAAATCCCTGAAAAA	47.6	40.0	58.0	55.1	1609-1615 bp	1.0	49.2
rpl16_p2	TCCAGAAAGAAAAACCTGGGG	TTTCCCGAAATCCCTGAAAA	47.6	40.0	58.0	55.1	1610-1616 bp	1.0	49.2
rpl16_p3	TCCAGAAAGAAAAACCTGGGG	TTTTCCCGAAATCCCTGAAA	47.6	40.0	58.0	55.1	1611-1617 bp	1.0	49.2
rpl16_p4	TCCAGAAAGAAAAACCTGGGG	TTTTTCCCGAAATCCCTGAA	47.6	40.0	58.0	55.1	1612-1618 bp	1.0	49.2
rpl16_p5	TCCAGAAAGAAAAACCTGGGG	TTTCCCGAAATCCCTGAAAAA	47.6	38.1	58.0	56.0	1610-1616 bp	1.0	47.7