Hotspot

ndhH

SSC · 117294-118475 · 1182 bp

Back to Chasmanthium

Pi 0.00592

MarkerSeek score 62.20

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 7
pi: 0.00592
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TCCCAGTAGGAGGGTCAAAA	TTTGCTGAGCCTCGTTTTGA	50.0	45.0	57.6	58.3	1371 bp	1.0	74.4
ndhH_p2	TCCCAGTAGGAGGGTCAAAA	TTTGCATTTTGCTGAGCCTC	50.0	45.0	57.6	57.6	1378 bp	1.0	73.4
ndhH_p3	TCCCAGTAGGAGGGTCAAAA	TTTTGCTGAGCCTCGTTTTG	50.0	45.0	57.6	57.5	1372 bp	1.0	73.1
ndhH_p4	TCCCAGTAGGAGGGTCAAAA	CATTTTGCTGAGCCTCGTTT	50.0	45.0	57.6	57.3	1374 bp	1.0	71.3
ndhH_p5	TCCCAGTAGGAGGGTCAAAA	TTGCTGAGCCTCGTTTTGAG	50.0	50.0	57.6	58.8	1370 bp	1.0	70.8