Hotspot

ndhC-trnV-UAC

LSC · 52353-53336 · 984 bp

Back to Chasmanthium

Pi 0.00312
MarkerSeek score 62.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
984
variable_sites
3
pi
0.00312
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAACCGGGGCTAAAAGTCC GGTTCGAATCCGTATAGCCC 55.0 55.0 58.0 57.9 1088-1110 bp 1.0 80.9
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAAGTCC AGCCCAATGCAGAAAAATGC 55.0 45.0 58.0 57.9 1181-1203 bp 1.0 80.6
ndhC-trnV-UAC_p3 CTAACCGGGGCTAAAAGTCC CGGTTCGAATCCGTATAGCC 55.0 55.0 58.0 58.3 1089-1111 bp 1.0 79.1
ndhC-trnV-UAC_p4 TAACCGGGGCTAAAAGTCCT GGTTCGAATCCGTATAGCCC 50.0 55.0 58.3 57.9 1087-1109 bp 1.0 78.5
ndhC-trnV-UAC_p5 TAACCGGGGCTAAAAGTCCT AGCCCAATGCAGAAAAATGC 50.0 45.0 58.3 57.9 1180-1202 bp 1.0 78.1