Hotspot

petN-trnC-GCA

LSC · 19307-20225 · 919 bp

Back to Chasmanthium

Pi 0.00225

MarkerSeek score 60.30

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 919
variable_sites: 2
pi: 0.00225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	CTGCAAATCCTTTACCCCCA	50.0	50.0	58.2	57.8	957-987 bp	1.0	79.6
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	TGCAAATCCTTTACCCCCAG	50.0	50.0	58.2	57.8	956-986 bp	1.0	79.6
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	ACTGCAAATCCTTTACCCCC	50.0	50.0	58.2	57.8	958-988 bp	1.0	79.6
petN-trnC-GCA_p4	AGCCCAAGCGAGACTTACTA	GCAAATCCTTTACCCCCAGT	50.0	50.0	58.2	57.8	955-985 bp	1.0	79.6
petN-trnC-GCA_p5	CCACTCCTCCCCCATACTAC	TGCAAATCCTTTACCCCCAG	60.0	50.0	58.3	57.8	1012-1042 bp	1.0	78.4