Hotspot

rps3

LSC · 80977-81651 · 675 bp

Back to Chasechloa

Pi 0.00741

MarkerSeek score 63.00

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 675
variable_sites: 5
pi: 0.00741
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TATCACAGGGCAGAATGGGA	TCAATCCAAGATTTCGATTCCCA	50.0	39.1	58.1	58.1	760 bp	1.0	54.5
rps3_p2	TATCACAGGGCAGAATGGGA	CAATCCAAGATTTCGATTCCCA	50.0	40.9	58.1	56.7	759 bp	1.0	53.6
rps3_p3	CTATCACAGGGCAGAATGGG	TCAATCCAAGATTTCGATTCCCA	55.0	39.1	57.7	58.1	761 bp	1.0	53.2
rps3_p4	AGGGGTTTTGGTTTGGGAAA	TCAATCCAAGATTTCGATTCCCA	45.0	39.1	57.7	58.1	805 bp	1.0	53.2
rps3_p5	TATCACAGGGCAGAATGGGA	TCCAAGATTTCGATTCCCAA	50.0	40.0	58.1	54.6	756 bp	1.0	52.7