Hotspot

rpoC2

LSC · 26907-31427 · 4521 bp

Back to Chasechloa

Pi 0.00929

MarkerSeek score 45.60

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4521
variable_sites: 42
pi: 0.00929
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	ATATGGGACCCGAGAAGGAG	ATGGAATCTTCCTCCAGGCT	55.0	50.0	58.0	58.1	327-351 bp	1.0	85.2
rpoC2_p2	ATATGGGACCCGAGAAGGAG	TTCCTCCAGGCTCCCATATT	55.0	50.0	58.0	57.8	319-343 bp	1.0	84.4
rpoC2_p3	GAACGAATATGGGACCCGAG	ATGGAATCTTCCTCCAGGCT	55.0	50.0	57.8	58.1	333-357 bp	1.0	84.1
rpoC2_p4	GAGAACGAATATGGGACCCG	ATGGAATCTTCCTCCAGGCT	55.0	50.0	57.8	58.1	335-359 bp	1.0	84.0
rpoC2_p5	ATATGGGACCCGAGAAGGAG	CTTCCTCCAGGCTCCCATAT	55.0	55.0	58.0	58.3	320-344 bp	1.0	84.0