Hotspot

psbT

LSC · 72233-72334 · 102 bp

Back to Chasechloa

Pi 0.0196

MarkerSeek score 63.80

Primer available yes

Label psbT

Feature Metadata

label_name: psbT
feature_type: gene
region: LSC
length_bp: 102
variable_sites: 2
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT_p1	TCTCATGTCCTTTCTTTGACTCT	CCTCTGGGGGACTTCTTACT	39.1	55.0	57.2	57.7	267 bp	1.0	48.0
psbT_p2	TCTCATGTCCTTTCTTTGACTCT	CCATTCGAAGAACATGGGGA	39.1	50.0	57.2	57.6	309 bp	1.0	46.6
psbT_p3	TCTCATGTCCTTTCTTTGACTCT	TCCATTCGAAGAACATGGGG	39.1	50.0	57.2	57.6	310 bp	1.0	46.6
psbT_p4	TGTCCTTTCTTTGACTCTTTTTCG	CCTCTGGGGGACTTCTTACT	37.5	55.0	57.9	57.7	262 bp	1.0	45.6
psbT_p5	TGAGAAACATCTCATGTCCTTTCT	CCTCTGGGGGACTTCTTACT	37.5	55.0	57.9	57.7	276 bp	1.0	45.6