Hotspot

rpl2-trnH

IRa · 139730-139784 · 55 bp

Back to Chasechloa

Pi 0.0182
MarkerSeek score 63.00
Primer available yes
Label rpl2-trnH

Feature Metadata

label_name
rpl2-trnH
feature_type
igs
region
IRa
length_bp
55
variable_sites
1
pi
0.0182
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-trnH_p1 GTTATCCTGCGCTTGGAAGA CTTTAGCTGGATAAGGGGCG 50.0 55.0 58.0 58.1 223 bp 1.0 79.2
rpl2-trnH_p2 GTTATCCTGCGCTTGGAAGA CGGATGTAGCCAAGTGGATC 50.0 55.0 58.0 58.1 205 bp 1.0 79.2
rpl2-trnH_p3 GTTATCCTGCGCTTGGAAGA TGGATCAAGGCAGTGGATTG 50.0 50.0 58.0 57.9 191 bp 1.0 79.2
rpl2-trnH_p4 GTTATCCTGCGCTTGGAAGA GGATCAAGGCAGTGGATTGT 50.0 50.0 58.0 57.9 190 bp 1.0 79.1
rpl2-trnH_p5 GTTATCCTGCGCTTGGAAGA GTGGATCAAGGCAGTGGATT 50.0 50.0 58.0 57.9 192 bp 1.0 79.1