Hotspot

ndhI-ndhA

SSC · 114173-114265 · 93 bp

Back to Chasechloa

Pi 0.043

MarkerSeek score 77.20

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 93
variable_sites: 4
pi: 0.043
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TGACCAATGTATCTTGCGGC	TGGACTTTACCCAGGATGAGA	50.0	47.6	58.6	57.8	281 bp	1.0	66.6
ndhI-ndhA_p2	TGACCAATGTATCTTGCGGC	ACCCAGGATGAGAATGGATCA	50.0	47.6	58.6	58.2	273 bp	1.0	66.4
ndhI-ndhA_p3	CGCGTATTGTTTGTTGACCA	TGGACTTTACCCAGGATGAGA	45.0	47.6	57.3	57.8	262 bp	1.0	66.2
ndhI-ndhA_p4	GCGCGTATTGTTTGTTGACC	TGGACTTTACCCAGGATGAGA	50.0	47.6	58.7	57.8	263 bp	1.0	66.2
ndhI-ndhA_p5	GGCGCGTATTGTTTGTTGAC	TGGACTTTACCCAGGATGAGA	50.0	47.6	58.7	57.8	264 bp	1.0	66.2