Hotspot

rpl32-trnL

SSC · 108176-108691 · 516 bp

Back to Chasechloa

Pi 0.035

MarkerSeek score 66.20

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 516
variable_sites: 18
pi: 0.035
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	CCAAAAGGTTTTTCTGGGCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.0	57.9	603-621 bp	1.0	73.5
rpl32-trnL_p2	ACCAAAAGGTTTTTCTGGGC	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.0	57.9	604-622 bp	1.0	73.5
rpl32-trnL_p3	GGTTTTTCTGGGCAACAAACA	TCCTAAGAGCAGCGTGTCTA	42.9	50.0	58.0	57.9	597-615 bp	1.0	73.3
rpl32-trnL_p4	CCAAAAGGTTTTTCTGGGCA	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.0	57.8	596-614 bp	1.0	73.0
rpl32-trnL_p5	CCAAAAGGTTTTTCTGGGCA	GCTTGAGCACTGCTTCCTAA	45.0	50.0	57.0	58.2	617-635 bp	1.0	72.9