Hotspot

rpl33-rps18

LSC · 67078-67346 · 269 bp

Back to Chasechloa

Pi 0.0262

MarkerSeek score 60.90

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 269
variable_sites: 7
pi: 0.0262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AGAATCGCCACAATACACCC	TGGATTTACGAAAGGGTTGCT	50.0	42.9	58.0	57.9	414-416 bp	1.0	72.2
rpl33-rps18_p2	CCACAATACACCCGGACAAT	TGGATTTACGAAAGGGTTGCT	50.0	42.9	57.9	57.9	407-409 bp	1.0	71.6
rpl33-rps18_p3	TGTCGTTATTGTCGCAAGCA	TGGATTTACGAAAGGGTTGCT	45.0	42.9	58.5	57.9	370-372 bp	1.0	68.8
rpl33-rps18_p4	AGAATCGCCACAATACACCC	TGGATTTACGAAAGGGTTGC	50.0	45.0	58.0	56.3	414-416 bp	1.0	68.0
rpl33-rps18_p5	TTGTCGTTATTGTCGCAAGC	TGGATTTACGAAAGGGTTGCT	45.0	42.9	57.4	57.9	371-373 bp	1.0	68.0