Hotspot

ycf3-trnS

LSC · 46109-46687 · 579 bp

Back to Chasechloa

Pi 0.0138

MarkerSeek score 51.70

Primer available yes

Label ycf3-trnS

Feature Metadata

label_name: ycf3-trnS
feature_type: igs
region: LSC
length_bp: 579
variable_sites: 8
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS_p1	GGGGGCTGGTATTTTTCCAA	AGTGGGTCGGTCTCAAAGTA	50.0	50.0	58.1	58.0	1723-1725 bp	1.0	80.4
ycf3-trnS_p2	GGGGGCTGGTATTTTTCCAA	CCTTTCCCCGACCTAAATCG	50.0	55.0	58.1	58.1	1616-1618 bp	1.0	80.1
ycf3-trnS_p3	CTTTGTTCTCAACGCCTCCT	AGTGGGTCGGTCTCAAAGTA	50.0	50.0	58.1	58.0	1613-1615 bp	1.0	79.9
ycf3-trnS_p4	TCTTTGTTCTCAACGCCTCC	AGTGGGTCGGTCTCAAAGTA	50.0	50.0	58.1	58.0	1614-1616 bp	1.0	79.9
ycf3-trnS_p5	CGAAGGGGGCTGGTATTTTT	AGTGGGTCGGTCTCAAAGTA	50.0	50.0	58.2	58.0	1727-1729 bp	1.0	79.6