Hotspot

atpH-atpF

LSC · 34485-34963 · 479 bp

Back to Chasechloa

Pi 0.00649

MarkerSeek score 63.40

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 479
variable_sites: 3
pi: 0.00649
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	TTATGGACTGGTTGTGGCAC	CGAATGGCCAGTGAGCTAAA	50.0	50.0	58.1	58.3	567-580 bp	1.0	79.9
atpH-atpF_p2	TGTGGCACTAGCGCTTTTAT	CGAATGGCCAGTGAGCTAAA	45.0	50.0	57.9	58.3	555-568 bp	1.0	79.9
atpH-atpF_p3	TTGTGGCACTAGCGCTTTTA	CGAATGGCCAGTGAGCTAAA	45.0	50.0	58.1	58.3	556-569 bp	1.0	79.8
atpH-atpF_p4	TTATGGACTGGTTGTGGCAC	TATTAAGCCCGAAACTCCCG	50.0	50.0	58.1	57.4	588-601 bp	1.0	77.1
atpH-atpF_p5	TGTGGCACTAGCGCTTTTAT	TATTAAGCCCGAAACTCCCG	45.0	50.0	57.9	57.4	576-589 bp	1.0	77.0