Hotspot

ndhF

SSC · 110682-112898 · 2217 bp

Back to Chamaesium

Pi 0.0162

MarkerSeek score 57.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 117
pi: 0.0162
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TACGTATCCGCCTAGCTTGT	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.3	58.3	2883-2997 bp	0.888889	78.2
ndhF_p2	CGCCTAGCTTGTCCACTTTT	GGGGGTAGGATCCGGTAAAT	50.0	55.0	58.5	58.3	2875-2989 bp	0.888889	77.0
ndhF_p3	TTACGTATCCGCCTAGCTTG	GGGGGTAGGATCCGGTAAAT	50.0	55.0	57.2	58.3	2884-2998 bp	0.888889	74.4
ndhF_p4	TACGTATCCGCCTAGCTTGT	GGGTAGGATCCGGTAAATCG	50.0	55.0	58.3	57.3	2881-2995 bp	0.888889	74.4
ndhF_p5	CCCACACTCTCTTCGGATGA	GGGGGTAGGATCCGGTAAAT	55.0	55.0	58.8	58.3	2919-2927 bp	0.333333	45.1