Hotspot

ccsA-ndhD

SSC · 116206-116431 · 226 bp

Back to Chamaesium

Pi 0.0517

MarkerSeek score 75.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 226
variable_sites: 37
pi: 0.0517
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TAGCACTTCTGGTCCTCACA	TGGACCGCGAGAGTTATTTG	50.0	50.0	58.0	58.0	858-868 bp	0.777778	76.9
ccsA-ndhD_p2	TAGCACTTCTGGTCCTCACA	GGACCGCGAGAGTTATTTGT	50.0	50.0	58.0	58.0	857-867 bp	0.777778	76.9
ccsA-ndhD_p3	TAGCACTTCTGGTCCTCACA	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.0	58.0	869-879 bp	0.777778	76.6
ccsA-ndhD_p4	TAGCACTTCTGGTCCTCACA	ACAAGCTGTTTAATGCCCCA	50.0	45.0	58.0	58.0	899-908 bp	0.666667	71.5
ccsA-ndhD_p5	TAGCACTTCTGGTCCTCACA	TATGCAGCTTCAACATCCCC	50.0	50.0	58.0	57.9	1362-1371 bp	0.444444	59.0