Hotspot

rpl32-trnL-UAG

SSC · 114276-115057 · 782 bp

Back to Chamaesium

Pi 0.0536

MarkerSeek score 83.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 782
variable_sites: 115
pi: 0.0536
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGTATTGGGCAGCCTTAAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.8	58.0	797-1015 bp	1.0	90.7
rpl32-trnL-UAG_p2	GGGTATTGGGCAGCCTTAAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.8	58.0	798-1016 bp	1.0	90.7
rpl32-trnL-UAG_p3	GGGTATTGGGCAGCCTTAAA	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	57.8	58.1	1279-1497 bp	1.0	90.1
rpl32-trnL-UAG_p4	AAAGGAAAGGGGTATTGGGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.8	58.0	806-1024 bp	0.777778	81.1
rpl32-trnL-UAG_p5	AAAGGAAAGGGGTATTGGGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.8	58.0	807-1025 bp	0.777778	81.1