Hotspot

rpl22-rps19

LSC · 84358-84427 · 70 bp

Back to Chamaesium

Pi 0.0478

MarkerSeek score 77.00

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 70
variable_sites: 11
pi: 0.0478
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	ATACGTGCAATTCCCTCGAC	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.1	58.0	1276-1303 bp	1.0	85.9
rpl22-rps19_p2	CGTAGGAACGTCCACGAATT	GGTTATCCTGCACTTGGGAG	50.0	55.0	58.0	58.0	590-611 bp	0.888889	82.2
rpl22-rps19_p3	CGTAGGAACGTCCACGAATT	GAAAAAGACCCACAACCCCT	50.0	50.0	58.0	57.9	613-634 bp	0.888889	81.6
rpl22-rps19_p4	CGTAGGAACGTCCACGAATT	AACCCTGTAGACCATCCACA	50.0	50.0	58.0	58.0	665-686 bp	0.888889	81.4
rpl22-rps19_p5	CGTAGGAACGTCCACGAATT	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	727-748 bp	0.888889	81.2