Hotspot

trnM-CAU-atpE

LSC · 52952-53164 · 213 bp

Back to Chamaesium

Pi 0.0408
MarkerSeek score 74.10
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
213
variable_sites
21
pi
0.0408
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 GGGTCTGTTTGGCACCTAAA ATTCACAAGAAGCCCAGCAA 50.0 45.0 58.0 58.0 778-793 bp 0.888889 83.8
trnM-CAU-atpE_p2 GGGTCTGTTTGGCACCTAAA ATTACCGGATCACGCCTCTA 50.0 50.0 58.0 58.0 942-957 bp 0.888889 83.1
trnM-CAU-atpE_p3 GGGTCTGTTTGGCACCTAAA TACCGGATCACGCCTCTATT 50.0 50.0 58.0 58.0 940-955 bp 0.888889 83.1
trnM-CAU-atpE_p4 GGGTCTGTTTGGCACCTAAA TATTACCGGATCACGCCTCT 50.0 50.0 58.0 58.0 943-958 bp 0.888889 83.1
trnM-CAU-atpE_p5 GGGTCTGTTTGGCACCTAAA TTACCGGATCACGCCTCTAT 50.0 50.0 58.0 58.0 941-956 bp 0.888889 83.1