Hotspot

trnE-UUC-trnT-GGU

LSC · 31299-32003 · 705 bp

Back to Chamaesium

Pi 0.0453

MarkerSeek score 82.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 705
variable_sites: 103
pi: 0.0453
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	724-979 bp	1.0	90.6
trnE-UUC-trnT-GGU_p2	GGATTCCCGGTCCAATCAAA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	1424-1796 bp	0.888889	86.0
trnE-UUC-trnT-GGU_p3	GACCCAGACCCAAGAAGAAC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	823-1078 bp	0.888889	86.0
trnE-UUC-trnT-GGU_p4	TAGGATTCCCGGTCCAATCA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	1426-1798 bp	0.888889	85.6
trnE-UUC-trnT-GGU_p5	GGAAATAAGGGATCCGGCAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.1	58.0	1089-1093 bp	0.444444	58.9