Hotspot

rps16-trnQ-UUG

LSC · 6253-7254 · 1002 bp

Back to Chamaesium

Pi 0.032

MarkerSeek score 75.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1002
variable_sites: 103
pi: 0.032
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CGCGCGATACACTTTGAATC	CCCGCCCAATCAAAAATTCC	50.0	50.0	58.0	58.0	1595-2042 bp	0.888889	85.2
rps16-trnQ-UUG_p2	ATCCTTCCAAAATGGCAGCA	CCCGCCCAATCAAAAATTCC	45.0	50.0	58.1	58.0	1667-2128 bp	0.888889	85.0
rps16-trnQ-UUG_p3	CGCGCGATACACTTTGAATC	CGAAAACTTACCGCAGCTTG	50.0	50.0	58.0	58.0	1881-2334 bp	0.888889	84.6
rps16-trnQ-UUG_p4	GCGCGATACACTTTGAATCG	CCCGCCCAATCAAAAATTCC	50.0	50.0	58.0	58.0	1594-2041 bp	0.777778	79.9
rps16-trnQ-UUG_p5	GCGCGATACACTTTGAATCG	CGAAAACTTACCGCAGCTTG	50.0	50.0	58.0	58.0	1880-2333 bp	0.777778	79.2