Hotspot

trnH-GUG-psbA

LSC · 78-416 · 339 bp

Back to Chamaesium

Pi 0.0866
MarkerSeek score 77.00
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
339
variable_sites
81
pi
0.0866
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.1 57.9 305-431 bp 0.777778 81.6
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 308-434 bp 0.777778 81.5
trnH-GUG-psbA_p3 GCCTTGATCCACTTGGCTAC CCTCTAGACCTAGCTGCTGT 55.0 55.0 58.6 57.9 310-436 bp 0.777778 77.7
trnH-GUG-psbA_p4 GCCTTGATCCACTTGGCTAC TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.6 57.9 313-439 bp 0.777778 77.6
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CCCTCTAGACCTAGCTGCTG 55.0 60.0 58.1 58.7 306-432 bp 0.777778 76.7