Hotspot

ycf3

LSC · 42791-44808 · 2018 bp

Back to Chamaerhodos

Pi 0.00152
MarkerSeek score 56.50
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
LSC
length_bp
2018
variable_sites
6
pi
0.00152
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 TGACGGATAGGGACAACAAA TGAGATCATTTTCTCACGAAAGG 45.0 39.1 56.5 56.9 2123-2142 bp 1.0 40.5
ycf3_p2 TTGACGGATAGGGACAACAA TGAGATCATTTTCTCACGAAAGG 45.0 39.1 56.5 56.9 2124-2143 bp 1.0 40.5
ycf3_p3 TTTGACGGATAGGGACAACA TGAGATCATTTTCTCACGAAAGG 45.0 39.1 56.5 56.9 2125-2144 bp 1.0 40.5
ycf3_p4 TGACGGATAGGGACAACAAA TGAGATCATTTTCTCACGAAAGGA 45.0 37.5 56.5 58.2 2123-2142 bp 1.0 40.5
ycf3_p5 TTGACGGATAGGGACAACAA TGAGATCATTTTCTCACGAAAGGA 45.0 37.5 56.5 58.2 2124-2143 bp 1.0 40.5