Hotspot

ycf1

SSC · 123509-129217 · 5709 bp

Back to Chamaerhodos

Pi 0.00112

MarkerSeek score 45.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5709
variable_sites: 15
pi: 0.00112
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCGTTTATTTGTGTTGGAATTCGA	TGGTCGAGACTAGTACCACA	33.3	50.0	57.7	57.1	300 bp	1.0	40.5
ycf1_p2	TCGTTTATTTGTGTTGGAATTCGA	TGGTCGAGACTAGTACCACAA	33.3	47.6	57.7	57.9	300 bp	1.0	40.5
ycf1_p3	CGTTTATTTGTGTTGGAATTCGA	TGGTCGAGACTAGTACCACA	34.8	50.0	56.4	57.1	299 bp	1.0	40.5
ycf1_p4	TCGTTTATTTGTGTTGGAATTCG	TGGTCGAGACTAGTACCACA	34.8	50.0	56.4	57.1	300 bp	1.0	40.5
ycf1_p5	CGTTTATTTGTGTTGGAATTCGA	TGGTCGAGACTAGTACCACAA	34.8	47.6	56.4	57.9	299 bp	1.0	40.5