Hotspot

ndhA

SSC · 119235-121544 · 2310 bp

Back to Chamaerhodos

Pi 0.0019

MarkerSeek score 58.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2310
variable_sites: 11
pi: 0.0019
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGACCATAATTCATGAACCCAGT	TGGGAGAAGTTGATCGTTGA	39.1	45.0	57.8	56.5	2449-2468 bp	1.0	42.8
ndhA_p2	TGACCATAATTCATGAACCCAGT	TGGGAGAAGTTGATCGTTGAA	39.1	42.9	57.8	57.2	2449-2468 bp	1.0	40.8
ndhA_p3	TGACCATAATTCATGAACCCAGT	ATGGGAGAAGTTGATCGTTGA	39.1	42.9	57.8	57.0	2450-2469 bp	1.0	40.4
ndhA_p4	TCATGAACCCAGTTACCATAGA	TGGGAGAAGTTGATCGTTGA	40.9	45.0	56.3	56.5	2439-2458 bp	1.0	40.4
ndhA_p5	TTCATGAACCCAGTTACCAT	TGGGAGAAGTTGATCGTTGA	40.0	45.0	54.2	56.5	2440-2459 bp	1.0	40.4