Hotspot

rpl32-trnL-UAG

SSC · 112952-113628 · 677 bp

Back to Chamaerhodos

Pi 0.00266

MarkerSeek score 53.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 677
variable_sites: 3
pi: 0.00266
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGAAAGGTTATTGGGCAGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	799-802 bp	1.0	79.4
rpl32-trnL-UAG_p2	GAAAGGTTATTGGGCAGCCT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	797-800 bp	1.0	79.4
rpl32-trnL-UAG_p3	AGGAAAGGTTATTGGGCAGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	800-803 bp	1.0	79.3
rpl32-trnL-UAG_p4	GAAAGGTTATTGGGCAGCCT	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	798-801 bp	1.0	79.3
rpl32-trnL-UAG_p5	GGCAGCCTTGAAAGCTTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	785-788 bp	1.0	78.6