Hotspot

ycf1-ndhF

SSC · 110005-110022 · 18 bp

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Pi 0.102

MarkerSeek score 80.00

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 18
variable_sites: 3
pi: 0.102
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	TCTGGATGGGAATCAAGAACA	CAAATATGTAGGGGGCGGAC	42.9	55.0	56.6	58.1	172-194 bp	1.0	65.4
ycf1-ndhF_p2	TCTGGATGGGAATCAAGAACA	TCAAATATGTAGGGGGCGGA	42.9	50.0	56.6	58.2	173-195 bp	1.0	64.7
ycf1-ndhF_p3	TCTGGATGGGAATCAAGAACAA	CAAATATGTAGGGGGCGGAC	40.9	55.0	57.3	58.1	172-194 bp	1.0	63.2
ycf1-ndhF_p4	TGGATGGGAATCAAGAACAAGA	CAAATATGTAGGGGGCGGAC	40.9	55.0	57.3	58.1	170-192 bp	1.0	63.2
ycf1-ndhF_p5	TGGATGGGAATCAAGAACAAGA	TCAAATATGTAGGGGGCGGA	40.9	50.0	57.3	58.2	171-193 bp	1.0	62.5