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psbE-petL

LSC · 64229-65463 · 1235 bp

Back to Chamaerhodos

Pi 0.00162

MarkerSeek score 61.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1235
variable_sites: 5
pi: 0.00162
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AAGTCGTATCTTGCTCAGGC	50.0	50.0	57.8	57.7	1348-1352 bp	1.0	77.3
psbE-petL_p2	ACGAATAACCAACCCGCAAT	AAGTCGTATCTTGCTCAGGC	45.0	50.0	58.2	57.7	1434-1438 bp	1.0	77.1
psbE-petL_p3	TGCTGACGAATAACCAACCC	AAGTCGTATCTTGCTCAGGC	50.0	50.0	58.2	57.7	1439-1443 bp	1.0	77.0
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AAGTCGTATCTTGCTCAGGC	55.0	50.0	58.2	57.7	1343-1347 bp	1.0	77.0
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	AAGTCGTATCTTGCTCAGGC	50.0	50.0	58.4	57.7	1341-1345 bp	1.0	75.7