Hotspot

psbF-psbE

LSC · 63968-63976 · 9 bp

Back to Chamaerhodos

Pi 0.0444

MarkerSeek score 70.00

Primer available yes

Label psbF-psbE

Feature Metadata

label_name: psbF-psbE
feature_type: igs
region: LSC
length_bp: 9
variable_sites: 1
pi: 0.0444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbF-psbE_p1	ACGGTAGGTACAGCTAGTCC	GTATTTGGAAGCCCTCGTCC	55.0	55.0	58.0	58.3	200 bp	1.0	77.3
psbF-psbE_p2	GGTAGGTACAGCTAGTCCGT	GTATTTGGAAGCCCTCGTCC	55.0	55.0	58.0	58.3	198 bp	1.0	77.3
psbF-psbE_p3	CTAGTCCGTGAACCGCTAAC	GTATTTGGAAGCCCTCGTCC	55.0	55.0	58.1	58.3	187 bp	1.0	77.0
psbF-psbE_p4	ACGGTAGGTACAGCTAGTCC	TGTATTTGGAAGCCCTCGTC	55.0	50.0	58.0	57.6	201 bp	1.0	76.8
psbF-psbE_p5	GGTAGGTACAGCTAGTCCGT	TGTATTTGGAAGCCCTCGTC	55.0	50.0	58.0	57.6	199 bp	1.0	76.8