Hotspot

petA-psbJ

LSC · 62467-63449 · 983 bp

Back to Chamaerhodos

Pi 0.000816

MarkerSeek score 60.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 983
variable_sites: 2
pi: 0.000816
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AAGGTTCAGTTGGCCGAAAT	CCGGATTAGGTTCATCTCTGT	45.0	47.6	58.0	56.9	1028-1036 bp	1.0	63.9
petA-psbJ_p2	TTGAGAAGGTTCAGTTGGCC	CCGGATTAGGTTCATCTCTGT	50.0	47.6	58.0	56.9	1033-1041 bp	1.0	63.8
petA-psbJ_p3	GATCCATTACGTGTCCAGGG	CCGGATTAGGTTCATCTCTGT	55.0	47.6	57.8	56.9	1118-1126 bp	1.0	61.9
petA-psbJ_p4	AAGGTTCAGTTGGCCGAAAT	TCCGGATTAGGTTCATCTCTGT	45.0	45.5	58.0	58.4	1029-1037 bp	1.0	61.9
petA-psbJ_p5	TTGAGAAGGTTCAGTTGGCC	TCCGGATTAGGTTCATCTCTGT	50.0	45.5	58.0	58.4	1034-1042 bp	1.0	61.8