Hotspot

psaI-ycf4

LSC · 59247-59684 · 438 bp

Back to Chamaerhodos

Pi 0.00183

MarkerSeek score 56.40

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 438
variable_sites: 2
pi: 0.00183
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	TACGTTCTGATCGCCAACTC	45.0	50.0	58.0	57.7	516-521 bp	1.0	78.7
psaI-ycf4_p2	GGGCTTAGTATTTCCGGCAA	TACGTTCTGATCGCCAACTC	50.0	50.0	58.0	57.7	525-530 bp	1.0	78.5
psaI-ycf4_p3	CGGCAATTGCAATGGTTTCT	TACGTTCTGATCGCCAACTC	45.0	50.0	57.9	57.7	511-516 bp	1.0	78.2
psaI-ycf4_p4	ATTTCCGGCAATTGCAATGG	CGTTCTGATCGCCAACTCAT	45.0	50.0	58.0	58.4	514-519 bp	1.0	78.0
psaI-ycf4_p5	ATTTCCGGCAATTGCAATGG	AAAAGAATGAGGGCCCAACC	45.0	50.0	58.0	58.4	580-585 bp	1.0	77.9