Hotspot

trnR-UCU-atpA

LSC · 9922-10526 · 605 bp

Back to Chamaerhodos

Pi 0.00496
MarkerSeek score 55.10
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
605
variable_sites
7
pi
0.00496
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 AGTGAGAAGCGTCCATTGTC AGACGTTTACCGAAGAAGCG 50.0 50.0 57.9 58.3 771-776 bp 1.0 77.7
trnR-UCU-atpA_p2 GAGTGAGAAGCGTCCATTGT AGACGTTTACCGAAGAAGCG 50.0 50.0 57.9 58.3 772-777 bp 1.0 77.7
trnR-UCU-atpA_p3 GTGAGAAGCGTCCATTGTCT AGACGTTTACCGAAGAAGCG 50.0 50.0 57.9 58.3 770-775 bp 1.0 77.7
trnR-UCU-atpA_p4 AGTGAGAAGCGTCCATTGTC AGAAGCGGAAACCCTTTTGA 50.0 45.0 57.9 57.6 758-763 bp 1.0 77.3
trnR-UCU-atpA_p5 GTGAGAAGCGTCCATTGTCT AGAAGCGGAAACCCTTTTGA 50.0 45.0 57.9 57.6 757-762 bp 1.0 77.3