Hotspot

trnS-GCU-trnG-UCC

LSC · 8357-8863 · 507 bp

Back to Chamaerhodos

Pi 0.00454

MarkerSeek score 56.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 507
variable_sites: 5
pi: 0.00454
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	549-580 bp	1.0	56.2
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	548-579 bp	1.0	56.2
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	AACCGAAAGACCCTTTAACT	50.0	40.0	58.0	54.5	586-617 bp	1.0	56.1
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	AACCGAAAGACCCTTTAACT	50.0	40.0	58.0	54.5	585-616 bp	1.0	56.1
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	AACCGAAAGACCCTTTAACT	50.0	40.0	58.2	54.5	640-671 bp	1.0	54.6