Hotspot

trnK-UUU-rps16

LSC · 4315-5080 · 766 bp

Back to Chamaerhodos

Pi 0.00157
MarkerSeek score 52.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
766
variable_sites
3
pi
0.00157
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 898-907 bp 1.0 78.9
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 897-906 bp 1.0 78.9
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.2 58.2 902-911 bp 1.0 77.3
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT CCTTGAAAAAGGCGCTCAAC 50.0 50.0 58.2 58.2 902-911 bp 1.0 77.3
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.2 58.2 901-910 bp 1.0 77.3