Hotspot

ndhE

Genome · 121510-121815 · 306 bp

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Pi 0

MarkerSeek score 20.80

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: Genome
length_bp: 306
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 20.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	TTCAATTGCTGAAGAGTCTT	TCAAATTAATAAGGAGCTGGTCA	35.0	34.8	52.7	55.6	375 bp	0.75	30.0
ndhE_p2	TTTCAATTGCTGAAGAGTCT	TCAAATTAATAAGGAGCTGGTCA	35.0	34.8	52.7	55.6	376 bp	0.75	30.0
ndhE_p3	TTTCAATTGCTGAAGAGTCTT	TCAAATTAATAAGGAGCTGGTCA	33.3	34.8	53.6	55.6	376 bp	0.75	30.0
ndhE_p4	TTCAATTGCTGAAGAGTCTT	TTCAAATTAATAAGGAGCTGGTCA	35.0	33.3	52.7	56.3	376 bp	0.75	30.0
ndhE_p5	TTTCAATTGCTGAAGAGTCT	TTCAAATTAATAAGGAGCTGGTCA	35.0	33.3	52.7	56.3	377 bp	0.75	30.0