Hotspot

ccsA-ndhD

Genome · 118912-119349 · 438 bp

Back to Chamaenerion

Pi 0.0426

MarkerSeek score 77.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: Genome
length_bp: 438
variable_sites: 34
pi: 0.0426
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGGCTTACATAGTTATGGTTCA	ATCCGGATTTCGTTCTTTCA	36.4	40.0	54.6	55.1	529-546 bp	1.0	49.1
ccsA-ndhD_p2	AGGCTTACATAGTTATGGTTCA	TCCGGATTTCGTTCTTTCAT	36.4	40.0	54.6	55.1	528-545 bp	1.0	49.1
ccsA-ndhD_p3	AGGCTTACATAGTTATGGTTCA	TCCGGATTTCGTTCTTTCATT	36.4	38.1	54.6	55.9	528-545 bp	1.0	49.1
ccsA-ndhD_p4	AGGCTTACATAGTTATGGTTCA	TGGGATTGGTATTTATCCGGA	36.4	42.9	54.6	56.2	543-560 bp	1.0	48.9
ccsA-ndhD_p5	AGGCTTACATAGTTATGGTTCA	TTGGGATTGGTATTTATCCGGA	36.4	40.9	54.6	56.9	544-561 bp	1.0	48.9