Hotspot

ndhF-rpl32

Genome · 116395-117266 · 872 bp

Back to Chamaenerion

Pi 0.0199

MarkerSeek score 62.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 872
variable_sites: 33
pi: 0.0199
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TACCATAGTAGCGGCATGGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	2033-2074 bp	1.0	85.8
ndhF-rpl32_p2	TACCATAGTAGCGGCATGGA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	2034-2075 bp	1.0	85.8
ndhF-rpl32_p3	GGCATGGATAAGAGCGGAAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	2021-2062 bp	1.0	85.6
ndhF-rpl32_p4	GGCATGGATAAGAGCGGAAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	2022-2063 bp	1.0	85.5
ndhF-rpl32_p5	TACCATAGTAGCGGCATGGA	AGAAAGACTCCCCATTCGGA	50.0	50.0	58.0	58.0	1853-1920 bp	1.0	85.2