Hotspot

atpB-rbcL

Genome · 57617-58361 · 745 bp

Back to Chamaenerion

Pi 0.00425

MarkerSeek score 61.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: Genome
length_bp: 745
variable_sites: 6
pi: 0.00425
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GATTTGAGCGATACGTCCCA	ATCTTTAACGCCGGCTTTGA	50.0	45.0	57.8	58.2	880-882 bp	1.0	77.3
atpB-rbcL_p2	TGATTTGAGCGATACGTCCC	ATCTTTAACGCCGGCTTTGA	50.0	45.0	57.8	58.2	881-883 bp	1.0	77.3
atpB-rbcL_p3	GATTTGAGCGATACGTCCCA	TCTTTAACGCCGGCTTTGAA	50.0	45.0	57.8	58.4	879-881 bp	1.0	75.6
atpB-rbcL_p4	TGATTTGAGCGATACGTCCC	TCTTTAACGCCGGCTTTGAA	50.0	45.0	57.8	58.4	880-882 bp	1.0	75.6
atpB-rbcL_p5	TGGACCAATGATTTGAGCGA	ATCTTTAACGCCGGCTTTGA	45.0	45.0	57.5	58.2	889-891 bp	1.0	75.2