Hotspot

psbC-trnS

Genome · 37016-37229 · 214 bp

Back to Chamaenerion

Pi 0.021

MarkerSeek score 66.80

Primer available yes

Label psbC-trnS

Feature Metadata

label_name: psbC-trnS
feature_type: igs
region: Genome
length_bp: 214
variable_sites: 9
pi: 0.021
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS_p1	CCTGTTCTTTCCATGACCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.2	58.2	258-267 bp	1.0	83.7
psbC-trnS_p2	CCTGTTCTTTCCATGACCCC	TGAAAACCGGCATAGTTCGT	55.0	45.0	58.2	57.8	294-303 bp	1.0	83.5
psbC-trnS_p3	CCTGTTCTTTCCATGACCCC	GATGGCTCCGGTCTTGAAAA	55.0	50.0	58.2	58.2	308-317 bp	1.0	83.2
psbC-trnS_p4	AGCTGCAGCAGGATTTGAAA	TGAAAACCGGCATAGTTCGT	45.0	45.0	58.4	57.8	337-346 bp	1.0	81.0
psbC-trnS_p5	AGCTGCAGCAGGATTTGAAA	GATGGCTCCGGTCTTGAAAA	45.0	50.0	58.4	58.2	351-360 bp	1.0	80.7