Hotspot

trnT-GGU-psbD

Genome · 33146-34584 · 1439 bp

Back to Chamaenerion

Pi 0.00418

MarkerSeek score 59.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: Genome
length_bp: 1439
variable_sites: 11
pi: 0.00418
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	55.0	50.0	58.1	57.7	1508-1603 bp	1.0	82.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GACCGGACCAACCTACAAAA	50.0	50.0	58.1	57.7	1511-1606 bp	1.0	81.8
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	TAGACCGGACCAACCTACAA	55.0	50.0	58.1	57.7	1510-1605 bp	1.0	81.6
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	TAGACCGGACCAACCTACAA	50.0	50.0	58.1	57.7	1513-1608 bp	1.0	81.4
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	GAGCAATAGACCGGACCAAC	55.0	55.0	58.1	58.3	1516-1611 bp	1.0	81.3