Hotspot

psbM-trnD

Genome · 30973-32072 · 1100 bp

Back to Chamaenerion

Pi 0.0105

MarkerSeek score 64.90

Primer available yes

Label psbM-trnD

Feature Metadata

label_name: psbM-trnD
feature_type: igs
region: Genome
length_bp: 1100
variable_sites: 18
pi: 0.0105
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD_p1	AAAACGCAGTCGGAACTAGA	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.2	58.5	1185-1228 bp	1.0	74.2
psbM-trnD_p2	AAACGCAGTCGGAACTAGAA	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.2	58.5	1184-1227 bp	1.0	74.2
psbM-trnD_p3	AACGCAGTCGGAACTAGAAT	TTCAATTGGTCAGAGCACCG	45.0	50.0	57.0	58.5	1183-1226 bp	1.0	72.4
psbM-trnD_p4	AAAAACGCAGTCGGAACTAGA	TTCAATTGGTCAGAGCACCG	42.9	50.0	57.9	58.5	1186-1229 bp	1.0	71.8
psbM-trnD_p5	AAAACGCAGTCGGAACTAGAA	TTCAATTGGTCAGAGCACCG	42.9	50.0	57.9	58.5	1185-1228 bp	1.0	71.8