Hotspot

atpH-atpI

Genome · 14681-15591 · 911 bp

Back to Chamaenerion

Pi 0.021

MarkerSeek score 61.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: Genome
length_bp: 911
variable_sites: 24
pi: 0.021
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATGGAGGGTCATCATTGA	45.0	44.4	58.0	52.2	937-985 bp	1.0	44.7
atpH-atpI_p2	AATAGAAGCAAGCCCGACAG	ATGGAGGGTCATCATTGA	50.0	44.4	58.0	52.2	971-1019 bp	1.0	44.5
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	ATGGAGGGTCATCATTGA	50.0	44.4	57.9	52.2	993-1041 bp	1.0	44.4
atpH-atpI_p4	TACCCTCTACAGCTTGTCCC	ATGGAGGGTCATCATTGA	55.0	44.4	58.1	52.2	1020-1068 bp	1.0	44.3
atpH-atpI_p5	GCGATACCCTCTACAGCTTG	ATGGAGGGTCATCATTGA	55.0	44.4	57.9	52.2	1024-1072 bp	1.0	44.3