Hotspot

rps16-trnQ

Genome · 6058-7497 · 1440 bp

Back to Chamaenerion

Pi 0.0127
MarkerSeek score 61.70
Primer available yes
Label rps16-trnQ

Feature Metadata

label_name
rps16-trnQ
feature_type
igs
region
Genome
length_bp
1440
variable_sites
35
pi
0.0127
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ_p1 CGCGTTGCTTTTTACCACAT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.0 58.1 1495-1518 bp 1.0 82.0
rps16-trnQ_p2 GAATAGAAAGTGGCGGGTGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1551-1574 bp 1.0 81.4
rps16-trnQ_p3 GAAAGTGGCGGGTGTAAGAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 1546-1569 bp 1.0 81.4
rps16-trnQ_p4 ATCATGTCTGTCAAGTCGCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 1513-1536 bp 1.0 79.4
rps16-trnQ_p5 AAAGTGGCGGGTGTAAGAAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 1545-1568 bp 1.0 79.1