Hotspot

trnK-rps16

Genome · 4170-4913 · 744 bp

Back to Chamaenerion

Pi 0.0188

MarkerSeek score 58.70

Primer available yes

Label trnK-rps16

Feature Metadata

label_name: trnK-rps16
feature_type: igs
region: Genome
length_bp: 744
variable_sites: 25
pi: 0.0188
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-rps16_p1	CAGTTCAGGATCAGTCGTGG	GCCGTACGAGGAGAAAACTT	55.0	50.0	58.0	57.9	1168-1189 bp	1.0	81.5
trnK-rps16_p2	GTTCAGGATCAGTCGTGGTC	GCCGTACGAGGAGAAAACTT	55.0	50.0	58.0	57.9	1166-1187 bp	1.0	81.5
trnK-rps16_p3	TGCTTTTTCCAGGCATTCCT	GCCGTACGAGGAGAAAACTT	45.0	50.0	58.0	57.9	1190-1211 bp	1.0	81.4
trnK-rps16_p4	ATGCAAAAACCCTTCTCCGT	GCCGTACGAGGAGAAAACTT	45.0	50.0	58.0	57.9	1416-1443 bp	1.0	81.2
trnK-rps16_p5	CGACATGCTGCTTTTTCCAG	GCCGTACGAGGAGAAAACTT	50.0	50.0	58.0	57.9	1198-1219 bp	1.0	81.2