Hotspot

ndhF

SSC · 111874-114102 · 2229 bp

Back to Chamaelirium

Pi 0.0194

MarkerSeek score 55.90

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 43
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCGGGTGAATGGAAAGGAAA	GCCGTGCCATCCCTATAATT	50.0	50.0	58.1	57.8	2955-2997 bp	1.0	80.6
ndhF_p2	GATCCGGGTGAATGGAAAGG	GCCGTGCCATCCCTATAATT	55.0	50.0	58.3	57.8	2958-3000 bp	1.0	78.7
ndhF_p3	ATCCGGGTGAATGGAAAGGA	GCCGTGCCATCCCTATAATT	50.0	50.0	58.7	57.8	2957-2999 bp	1.0	75.6
ndhF_p4	CCGGGTGAATGGAAAGGAAA	TGCCGTGCCATCCCTATAAT	50.0	50.0	58.1	58.9	2956-2998 bp	1.0	74.7
ndhF_p5	TCCGGGTGAATGGAAAGGAA	GCCGTGCCATCCCTATAATT	50.0	50.0	58.9	57.8	2956-2998 bp	1.0	73.8