Hotspot

infA

LSC · 80641-80874 · 234 bp

Back to Chamaelirium

Pi 0.0128

MarkerSeek score 45.10

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 234
variable_sites: 3
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	AATGCTGCATCTCTTCCGAG	TTTTCAAGCGTATCAGCCGA	50.0	45.0	58.0	57.9	1170-1176 bp	1.0	80.7
infA_p2	TTAGGAGGTCGACACCCATT	TTTTCAAGCGTATCAGCCGA	50.0	45.0	58.1	57.9	1254-1260 bp	1.0	80.7
infA_p3	TTTAGGAGGTCGACACCCAT	TTTTCAAGCGTATCAGCCGA	50.0	45.0	58.1	57.9	1255-1261 bp	1.0	80.7
infA_p4	AATGCTGCATCTCTTCCGAG	GTTTTCAAGCGTATCAGCCG	50.0	50.0	58.0	57.8	1171-1177 bp	1.0	80.2
infA_p5	CTTGAGCAGCATAGGGTGTT	TTTTCAAGCGTATCAGCCGA	50.0	45.0	57.9	57.9	1079 bp	1.0	79.7