Hotspot

ndhF-rpl32

SSC · 114103-114862 · 760 bp

Back to Chamaelirium

Pi 0.0286

MarkerSeek score 69.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 760
variable_sites: 21
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGTAAGTGGAAGAAAGGGT	GGTGGAAACCGATTTAGCCA	45.0	50.0	54.6	58.2	920-932 bp	1.0	55.4
ndhF-rpl32_p2	GGAAGAAAGGGTATTATCCATGC	GGTGGAAACCGATTTAGCCA	43.5	50.0	57.0	58.2	912-924 bp	1.0	51.1
ndhF-rpl32_p3	AGTGGAAGAAAGGGTATTATCCA	GGTGGAAACCGATTTAGCCA	39.1	50.0	56.7	58.2	915-927 bp	1.0	48.2
ndhF-rpl32_p4	CTGTAAGTGGAAGAAAGGGTA	GGTGGAAACCGATTTAGCCA	42.9	50.0	54.6	58.2	920-932 bp	1.0	47.4
ndhF-rpl32_p5	CTGTAAGTGGAAGAAAGGGT	AGAAAGGGCTTTTACTGCAGT	45.0	42.9	54.6	57.8	899-911 bp	1.0	47.0