Hotspot

rpl36-infA

LSC · 80535-80640 · 106 bp

Back to Chamaelirium

Pi 0.066

MarkerSeek score 71.80

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 106
variable_sites: 7
pi: 0.066
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	AATCACTCTAATCCGTCCGC	CGCAACAAAGATTCGAACGA	50.0	45.0	57.5	57.4	208 bp	1.0	74.0
rpl36-infA_p2	AATCACTCTAATCCGTCCGC	TCGCAACAAAGATTCGAACG	50.0	45.0	57.5	57.4	209 bp	1.0	74.0
rpl36-infA_p3	AATCACTCTAATCCGTCCGC	TTCGCAACAAAGATTCGAACG	50.0	42.9	57.5	58.1	210 bp	1.0	69.8
rpl36-infA_p4	AATCACTCTAATCCGTCCGC	CGCAACAAAGATTCGAACGAT	50.0	42.9	57.5	57.9	208 bp	1.0	69.8
rpl36-infA_p5	CCTACGGATCAGTCGACATT	CGCAACAAAGATTCGAACGA	50.0	45.0	56.8	57.4	187 bp	1.0	69.2